Candida albicans is one of the most common opportunistic fungi which cause oral cavity infections in humans. Most anti-fungal drugs posse side effects and may have an undesirable taste. Ginger is one of the oldest herbal products used in traditional medicine which has known antimicrobial effects and is used in the manufacture of Vi-One mouthwash. The present study was designed to investigate the antifungal effects of Vi-One Mouthwash. In this experimental study, the susceptibility of Candida albicans (PTCC 5027) to Vi-One Ginger mouthwash was evaluated in comparison with nystatin using disk diffusion method. One way analysis of variance (ANOVA) test was employed to evaluate the findings and P-values less than 0.05 were considered as significant. The data of the present study showed that the diameter of the inhibition zone in the nystatin group was 8.04 mm while, in the vi-one mouthwash group it was 1.16 mm. The difference between examined groups was significantly different (ANOVA, P-value=0.0001).  According to the findings, vi-one ginger mouthwash exhibited a very weak antifungal effect in comparison with nystatin in the laboratory environment.