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A High-Performance Liquid Chromatographic Assay for the Determination of Losartan in Plasma
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Hassan Jalalizadeh   , Effat Souri , Hassan Farsam , Mehdi Ansari |
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Abstract: (1599 Views) |
| A rapid and sensitive HPLC method was developed for determination of losartan in plasma. Losartan was extracted from plasma by a two-step extraction procedure using chloroform as extracting solvent in acidic medium. HPLC analysis was performed on a cyano reversed-phase column using phosphate buffer (pH 4.3), acetonitrile (750:250, v/v) as mobile phase with a flow rate of 0.9 mL/min. Sodium diclofenac was selected as internal standard. Excellent linearity between the peak area ratios and losartan concentrations over the range of 2-200 ng/mL of plasma was observed. The limit of determination with UV detection at 225 nm, with a CV < 5% was 2 ng/mL in 500 L of plasma sample. The assay was rapid, safe and reliable for use in pharmacokinetic studies of losartan in human being. |
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Full-Text [PDF 327 kb]
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Article Type: Research Article |
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