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In Vitro Lipid Peroxidation Inhibitory and Antimicrobial Activity of Phyllanthus Niruri (Euphorbiaceae) Extract
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Yerra Rajeshwar   , Rayees Ahmad , A. Shyam Sunder , J. Devilal , Malaya Gupta , Upal Kanti Mazumder |
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Abstract: (1643 Views) |
| The present work was designed to evaluate the in vitro lipid peroxidation inhibitory and antimicrobial activities of the methanol extract of Phyllanthus niruri (MEPN) (Family: Euphorbiaceae) . Lipid peroxidation was measured by the optical density of the prepared solutions (10-320 µg/ml) and then the percent inhibition was calculated. Ascorbate/FeSO 4 -induced peroxidation was inhibited by standard antioxidants such as L-ascorbic acid, Quercetin and MEPN. Moreover, the percent inhibition of the methanol extract was increased in a concentration-dependent manner. IC 50 value for the MEPN, L-ascorbic acid and Quercetin for lipid peroxidation was found to be 62. 5 µg/ml, 41 µg/ml and 19.75 µg/ml respectively. The antimicrobial activity of MEPN was determined by disc diffusion method with various gram positive and gram-negative microorganisms. The MEPN showed strong antimicrobial activity against Bacillus pumillus 8241, Bacillus cereus , Escherichia Coli 54B and Vibrae Cholera at a concentration of 750 m g/ml/disc. However, its activity against Staphylococcus aureus ML 152 and Vibrae cholera 14035 was less significant. The antimicrobial activity of the extract was compared with the standard drug, Chloramphenicol at a concentration of 10 m g/ml/disc. The results obtained in the present investigation clearly suggest that MEPN can be a potential source of natural antioxidant and antimicrobial agent. |
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Article Type: Research Article |
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