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en Phenolic contents, In Vitro antioxidant, and In Vivo anti-inflammatory studies of aqueous extract from Pituranthos scoparius (Coss. & Dur.) growing in Algeria Other پژوهشي Research Article The present study aims to estimate the total phenolics, flavonoids and tannins contents then to investigate both in vitro antioxidant capacities models and topical anti-inflammatory activities of aqueous extract (AqE) from the stems of Pituranthos scoparius, growing in Algeria. Total polyphenol contents were determined using Folin Ciocalteu's reagent; flavonoids were quantified employing the AlCl3 and method tannins using haemoglobin precipitation test. The in vitro antioxidant properties was assessed using different models, including DPPH-scavenging assay, lipid peroxidation, OH˙ scavenging ability, iron chelation, and reducing power activities. In vivo, the topical anti-inflammatory effect of this extract was determined using croton oil-induced edema in mouse model. The results revealed that aqueous extract presented a high total phenolic and tannins contents with values of 150.89 ± 0.68 mg GAE (gallic acid equivalent)/g and 71.24 ± 0.09 mg TAE (tannic acid equivalent)/g dry extract, respectively. This extract show an essential effect toward DPPH-scavenging assay, lipid peroxidation inhibition and chelating effect, with 96.19 ± 0.00 µg/mL, 91.53 ± 0.98 % and 36.30 ± 0.00 µg/mL EC50, respectively. In a similar fashion, this extract exhibited an excellent topical anti-inflammatory activity value of 80.30 ± 5.91 % regardless of the dose of 2 mg/ear. This study indicates that the aqueous extract from Pituranthos scoparius has potent antioxidant and anti-inflammatory effects and may prove to be of latent health benefit as well as supplementary sources for natural antioxidants and anti-inflammatory drugs. Pituranthos scoparius, Aqueous extract, Anti-inflammatory activity, Antioxidant activity, Phenolic compounds. 1 7 http://ijpt.iums.ac.ir/browse.php?a_code=A-10-179-1&slc_lang=en&sid=1 KARBAB AHLEM karbabal2@gmail.com 10031947532846002118 10031947532846002118 Yes Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria. CHAREF NOUREDDIN charefnr@univ-setif.dz 10031947532846002119 10031947532846002119 No Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria. ARRAR LEKHMICI lekharrar@univ-setif.dz 10031947532846002120 10031947532846002120 No Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria.