en Toxicology پژوهشي Research Article <div style="text-align: justify;"><strong>ABSTRACT </strong><br> For a long period, ethno medicinal plants have been a valuable source of natural products for maintaining human health, especially in the last decade, with more intensive studies for natural therapies. The use of ethno medicinal plant for pharmaceutical purposes has gradually increased in Iran.&nbsp;<em>Gundelia tournefortii </em>has been used as an antibacterial, anti-fungal, antipyretic, anti-inflammatory, and antioxidant agent in Iran. In the recent examination, the testicular protective effect of <em>G. tournefortii </em>aerial parts aqueous extract on diabetic mice has been evaluated. Seventy mice were used and diabetes was induced by administration of 150 mg/kg of alloxan monohydrate intraperitoneally in 60 mature male mice and they were randomly divided into six groups. The treatment groups received glibenclamide 10 mg/kg and 5, 10, 20 and 40 mg/kg of <em>G. tournefortii </em>through gavage for 20 days. Also, one group was considered as the non-diabetic control. At 20^th day, the mice were killed, dissected, then blood and testis samples were collected for biochemical and stereological parameters analysis. The data were analyzed by SPSS-21 software. <em>G. tournefortii</em> at all doses (especially GT40) and glibenclamide significantly (p&le;0.05) ameliorated the concentrations of fasting blood glucose, testosterone, superoxide dismutase, catalase, glutathione reductase, and glutathione peroxidase. Also, multiple doses of <em>G. tournefortii</em> (especially GT40) and glibenclamide increased the weight and volume of the testis, the volumes of the tubule and interstitial tissue, the length and diameter of the tubule, the height of the germinal epithelium, and the number of the Leydig cell compared to the diabetic untreated group. According to the obtained results, <em>G. tournefortii </em>aerial parts aqueous extract can regulate the concentrations of biochemical parameters and inhibit testicular damages in alloxan monohydrate induced diabetic mice. It seems that <em>G. tournefortii </em>can be offered as a testicular protective supplement or drug for prevention, control, and treatment of testicular toxicity in diabetic patients.</div> Gundelia tournefortii, Aqueous extract, Testicular toxicity, Alloxan monohydrate. 1 9 http://ijpt.iums.ac.ir/browse.php?a_code=A-10-120-4&slc_lang=en&sid=1 SAMANEH GOORANI samanegoorani@ut.ac.ir 10031947532846001678 10031947532846001678 No Department of Toxicology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Mohammad KAZEM KOOHI mkkoohi@yahoo.com 10031947532846001679 10031947532846001679 No Department of Toxicology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. NILOOFAR SEYDI n.seydi@yahoo.com 10031947532846001680 10031947532846001680 No Department of Clinical Science, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran. AKRAM ZANGENEH akramzangeneh@yahoo.com 10031947532846001681 10031947532846001681 Yes Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran. MOHAMMAD MAHDI ZANGENEH m.mahdizangeneh@gmail.com 10031947532846001682 10031947532846001682 No Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran.