en Toxicology پژوهشي Research Article <strong>ABSTRACT </strong><br> Hepatotoxicity is the most common disorder with severe effects on quality of life. <em>Tragopogon graminifolius</em> DC. has been used in Iran as an astringent and bleeding inhibitor, wound healer and gastro-protector agent. To our knowledge, there are little evidence at hand on hepatoprotective activity of<em> T. graminifolius</em>. The present study was carried out to assess hepatoprotective property of <em>T. graminifolius</em> ethanolic extract on carbon tetrachloride (CCl₄) induced hepatotoxicity in mice for 45 consecutive days. Fifty male mice were divided into five groups (n=10). Group 1 (control) received 1 ml/kg olive oil intraperitoneally (i.p.) and distilled water orally; Group 2 (untreated) received CCl₄ (50% in olive oil, 1 ml/kg; i.p.); Groups 3, 4 and 5 received CCl₄ and 30, 90 and 270 mg/kg of <em>T. graminifolius</em> (T30, T90 and T270), respectively. At 45^th day, the mice were killed, dissected, then blood and liver samples were collected for biochemical and stereological parameters analysis. Biochemically, <em>T. graminifolius</em> at all doses (especially T270) could significantly (<em>p&le;</em>0.05) reduce the raised levels of cholesterol, low density lipoprotein (LDL), triglyceride, alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transferase (GGT), total and conjugated bilirubin, and increase the decreased levels of high density lipoprotein (HDL), total protein, albumin, superoxide dismutase (SOD) and catalase (CAT). Stereologically, different doses of <em>T. graminifolius</em> (especially T270) decreased the weight and volume of the hepatic structures as compared to the untreated group significantly (p&le;0.05). The obtained results indicate that<em> T. graminifolius </em>ethanolic extract, potential applications as a therapeutical supplement or medicine, after confirming clinical trial studies.<br> <br> <br> &nbsp; Tragopogon graminifolius DC., ethanolic extract, hepatoprotective property, carbon tetrachloride 1 8 http://ijpt.iums.ac.ir/browse.php?a_code=A-10-120-2&slc_lang=en&sid=1 SAMANEH GOORANI samanegoorani@ut.ac.ir 10031947532846001617 10031947532846001617 No Department of Toxicology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. MOHAMMAD KAZEM KOOHI mkkoohi@yahoo.com 10031947532846001618 10031947532846001618 No Department of Toxicology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. AKRAM ZANGENEH akramzangeneh@yahoo.com 10031947532846001619 10031947532846001619 No Department of Clinical Science, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran. FARIBA HOSSEINI fariba.hosseini2010@gmail.com 10031947532846001620 10031947532846001620 No Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran. MOHAMMAD MAHDI ZANGENEH m.mahdizangeneh@gmail.com 10031947532846001621 10031947532846001621 Yes Department of Clinical Science, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.