en Chemotherapy, Antibiotics and Gene Therapy پژوهشي Research Article <div style="text-align: justify;">In past decades, aminoglycosides have been commonly used to treat gram-negative infections as well as multi-drug resistant tuberculosis strains. However, in recent years, intrinsic, adaptive and acquired resistances have been raised against aminoglycosides which limits the uptake of these antibiotics. Acquired resistance to <em>Acinetobacter baumannii</em> responsible for nosocomial infection against aminoglycosides, has been led to a medical dilemma. In the present study, we aimed to investigate the prevalence rate of <em>A. baumannii</em> resistance to aminoglycosides using a meta-analysis and systematic review. International databases of Scopus, PubMed, Web of Science and Google Scholar, as well as national databases, include SID, Magiran, IranDoc, and IranMedex, were searched carefully and 62 articles published during years 1993 and 2016 were selected. After data extraction, random-effects model was used for analysis. Also, data heterogeneity was assessed using the I2 index and the final statistical analysis was done using STATA and R software. The total sample size of 28,055 extracted from the chosen articles and entered the meta-analysis process. The drug resistance value of <em>A. baumannii</em> isolates to various antibiotics was determined as follows: Amikacin 69% (57% to 81%), Tobramycin61% (52% to 71%), Netilmicin 35% (11% to 59%) and Gentamicin 68% (57% to 80%). The highest and lowest sensitivity of <em>A. baumannii</em> was considered against Netilmicin 57% (32% to 82%) and Gentamicin 26% (12% to 39%), respectively. According to our findings, the drug-resistance rate of <em>A. baumannii</em> clinical isolates to aminoglycosides, especially Amikacin, Tobramycin, and Gentamicin are relatively high. So, Gentamicin and Amikacin are not recommended as first-line treatment of <em>A. baumannii</em> isolates.<br> &nbsp;</div> Drug- resistance, Aminoglycosides, Acinetobacter baumannii 1 11 http://ijpt.iums.ac.ir/browse.php?a_code=A-10-94-1&slc_lang=en&sid=1 MOHAMMAD YOUSEF ALIKHANI alikhani@umsha.ac.ir 10031947532846001553 10031947532846001553 Yes Department of Microbiology and Brucellosis Research Center, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran ALI NAZARI nazari-a@medilam.ac.ir 10031947532846001554 10031947532846001554 No MD, Assistant professor, Department of Infectious Disease, School of Medicine, Ilam university of medical sciences, Ilam, Iran. KOUROSH SAYEHMIRI kouresh_sayehmiri@yahoo.com 10031947532846001555 10031947532846001555 No Department of social Medicine, Ilam University of Medical Sciences, Ilam, Iran FATEMEH SAYEHMIRI fsayehmiri@yahoo.com 10031947532846001556 10031947532846001556 No student research committee, Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran MANOOCHEHR KARAMI ma.karami@umsha.ac.ir 10031947532846001557 10031947532846001557 No Department of Epidemiology, Hamadan University of Medical Sciences, Hamadan, Iran JALAL GHADERKHANI jalalghaderkhani@gmail.com 10031947532846001558 10031947532846001558 No Department of Microbiology and Brucellosis Research Center, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran