en پژوهشي Research Article Normal 0 false false false MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;} The stem bark of Oroxylum indicum ( Fam. Bignoniaceae) was extracted with methanol. The methanol extract was fractionated with pet ether, chloroform, hexane and carbon tetrachloride using modified Kupchan partitioning method. Extensive chromatographic separation and purification with the organic solvent was done. One compound was extracted from the n-hexane fraction of stem bark of O. indicum which was then confirmed as flavonoid type of compound by using 1 H NMR spectrometric analysis. Three fractions (hexane, carbon tetrachloride, chloroform), obtained from methanolic crude extract, were tested for antimicrobial activity by standard disc diffusion method. The extracts were found to show antimicrobial activities towards the gram-positive and gram-negative bacteria, and some fungi . The results were compared with same microbial strains against a standard antibiotic disc of ampicillin (10 m g/disc). 25 0 http://ijpt.iums.ac.ir/browse.php?a_code=A-10-100-208&slc_lang=en&sid=1 M. Kaisarul Islam 1003194753284600705 1003194753284600705 No I. Zahan Eti 1003194753284600706 1003194753284600706 No J. Ahmed Chowdhury 1003194753284600707 1003194753284600707 No