en پژوهشي Research Article A rapid and sensitive HPLC method was developed for determination of losartan in plasma. Losartan was extracted from plasma by a two-step extraction procedure using chloroform as extracting solvent in acidic medium. HPLC analysis was performed on a cyano reversed-phase column using phosphate buffer (pH 4.3), acetonitrile (750:250, v/v) as mobile phase with a flow rate of 0.9 mL/min. Sodium diclofenac was selected as internal standard. Excellent linearity between the peak area ratios and losartan concentrations over the range of 2-200 ng/mL of plasma was observed. The limit of determination with UV detection at 225 nm, with a CV < 5% was 2 ng/mL in 500 L of plasma sample. The assay was rapid, safe and reliable for use in pharmacokinetic studies of losartan in human being. 18 0 http://ijpt.iums.ac.ir/browse.php?a_code=A-10-100-28&slc_lang=en&sid=1 Hassan Jalalizadeh 100319475328460060 100319475328460060 No Effat Souri 100319475328460061 100319475328460061 No Hassan Farsam 100319475328460062 100319475328460062 No Mehdi Ansari 100319475328460063 100319475328460063 No