en پژوهشي Research Article Gum of Butea monosperma is used to treat microbial and fungal infections in folk medicine. To validate this use, the in- vitro antimicrobial activity of petroleum ether and alcoholic extract of Butea monosperma gum was evaluated against various microbial strains such as Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Salmonella typhimurium , Pseudomonas aeuriogenosa, Escherichia coli, Candida albicans and Saccharomyces cerevisiae by using disc diffusion method. Minimum inhibitory concentration (MIC) was determined by agar dilution technique. Both extracts showed significant inhibition against reference gram positive bacteria and fungal strains. MIC value of petroleum ether extract against gram positive and fungal strains was 300 m g / ml and that of alcoholic extract was 200 m g / ml. No extract showed inhibitions against gram negative bacteria. 21 0 http://ijpt.iums.ac.ir/browse.php?a_code=A-10-100-172&slc_lang=en&sid=1 Shailendra S. Gurav 1003194753284600566 1003194753284600566 No Vijay D. Gulkari 1003194753284600567 1003194753284600567 No Nandkishore J. Duragkar 1003194753284600568 1003194753284600568 No Arun T. Patil 1003194753284600569 1003194753284600569 No