RT - Journal Article T1 - A High-Performance Liquid Chromatographic Assay for the Determination of Losartan in Plasma JF - iums-ijpt YR - 2003 JO - iums-ijpt VO - 2 IS - 1 UR - http://ijpt.iums.ac.ir/article-1-18-en.html SP - 18 EP - 0 AB - A rapid and sensitive HPLC method was developed for determination of losartan in plasma. Losartan was extracted from plasma by a two-step extraction procedure using chloroform as extracting solvent in acidic medium. HPLC analysis was performed on a cyano reversed-phase column using phosphate buffer (pH 4.3), acetonitrile (750:250, v/v) as mobile phase with a flow rate of 0.9 mL/min. Sodium diclofenac was selected as internal standard. Excellent linearity between the peak area ratios and losartan concentrations over the range of 2-200 ng/mL of plasma was observed. The limit of determination with UV detection at 225 nm, with a CV < 5% was 2 ng/mL in 500 L of plasma sample. The assay was rapid, safe and reliable for use in pharmacokinetic studies of losartan in human being. LA eng UL http://ijpt.iums.ac.ir/article-1-18-en.html M3 ER -