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Showing 2 results for NIKOUI
Sattar Ostadhadi, Mohsen Rashidi, Samira Zolfaghari, Jalal Mardaneh, Vahid Nikoui,
Volume 14, Issue 2 (8-2016)
Abstract
Appearance of bacteria resistant to antibacterial agents puts physicians in trouble and threatens the health of the world. The rapid development of bacterial resistance in Escherichia coli to ciprofloxacin makes difficult the treatment of infectious diseases. So, detection of the locations of possible mutations in gyrase A gene ( gyrA ) in these mutants is very important to determine the mechanism of this resistance. In the present study, ciprofloxacin-resistant mutants were isolated from medium containing ciprofloxacin. Polymerase chain reaction (PCR) was used to amplify the gyrA gene in these mutants and DNA sequencing was used to determine the location of mutation in this gene. Results showed that the most of ciprofloxacin-resistant mutants contain mutations in quinolone resistance- determining region (QRDR) of A subunit of DNA gyrase and specially at serine 83. However, mutations outside of this region were also found at tyrosine 50 and alanine 119. In conclusion, this study confirms that mutation in serine 83 of QRDR in A subunit of DNA gyrase is the main cause of resistance to ciprofloxacin in E. coli.
Abid Hayat, Muhammad Abdullah, Farid Ullah Shah, Mughal Qayum, Abdul Wahab, Muhammad Imran Khan, Bashir Ahmad, Vahid Nikoui,
Volume 17, Issue 1 (6-2019)
Abstract
Opium dependence is one of the serious and multidimensional problems. Millions of people are opium addicts throughout the world. The aim of this study was to determine the risk factors causing relapse in opium addicts in Internally Displaced People (IDPs). This experiment was conducted in the Drug Detoxification and Health Welfare research center, Bannu, KPK, Pakistan. Sociodemographics characteristics of IDPs were studied in this retrospective cross-sectional study. Questionnaire was specifically designed and total 41 relapsed individual’s histories of post treated IDPs were studied. Percentage of factors causing relapse in IDPs included stress in 36.59% individuals, family conflicts 19.51%, friends 12.20%, work load stress 09.76%, body aches 07.31%, sexual satisfaction 09.76% and fun 04.87%. Average time of relapse in IDPs was 6 months. Results revealed that stress was the most notorious factor directing IDPs towards relapse. It is concluded that attention must be paid on the crucial factors of stress to avoid relapse associated with opium dependence such as, family conflicts, personal, occupational and economical status.
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