:: Volume 17, Issue 1 (6-2019) ::
3 2019, 17(1): 1-7 Back to browse issues page
Phenolic contents, In Vitro antioxidant, and In Vivo anti-inflammatory studies of aqueous extract from Pituranthos scoparius (Coss. & Dur.) growing in Algeria
Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria.
Abstract:   (2050 Views)

The present study aims to estimate the total phenolics, flavonoids and tannins contents then to investigate both in vitro antioxidant capacities models and topical anti-inflammatory activities of aqueous extract (AqE) from the stems of Pituranthos scoparius, growing in Algeria. Total polyphenol contents were determined using Folin Ciocalteu's reagent; flavonoids were quantified employing the AlCl3 and method tannins using haemoglobin precipitation test. The in vitro antioxidant properties was assessed using different models, including DPPH-scavenging assay, lipid peroxidation, OH˙ scavenging ability, iron chelation, and reducing power activities. In vivo, the topical anti-inflammatory effect of this extract was determined using croton oil-induced edema in mouse model. The results revealed that aqueous extract presented a high total phenolic and tannins contents with values of 150.89 ± 0.68 mg GAE (gallic acid equivalent)/g and 71.24 ± 0.09 mg TAE (tannic acid equivalent)/g dry extract, respectively. This extract show an essential effect toward DPPH-scavenging assay, lipid peroxidation inhibition and chelating effect, with 96.19 ± 0.00 µg/mL, 91.53 ± 0.98 % and 36.30 ± 0.00 µg/mL EC50, respectively. In a similar fashion, this extract exhibited an excellent topical anti-inflammatory activity value of 80.30 ± 5.91 % regardless of the dose of 2 mg/ear. This study indicates that the aqueous extract from Pituranthos scoparius has potent antioxidant and anti-inflammatory effects and may prove to be of latent health benefit as well as supplementary sources for natural antioxidants and anti-inflammatory drugs.

Keywords: Pituranthos scoparius, Aqueous extract, Anti-inflammatory activity, Antioxidant activity, Phenolic compounds.

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