:: Volume 2, Issue 1 (5-2003) ::
3 2003, 2(1): 18-0 Back to browse issues page
A High-Performance Liquid Chromatographic Assay for the Determination of Losartan in Plasma
Hassan Jalalizadeh, Effat Souri, Hassan Farsam, Mehdi Ansari
Abstract:   (819 Views)
A rapid and sensitive HPLC method was developed for determination of losartan in plasma. Losartan was extracted from plasma by a two-step extraction procedure using chloroform as extracting solvent in acidic medium. HPLC analysis was performed on a cyano reversed-phase column using phosphate buffer (pH 4.3), acetonitrile (750:250, v/v) as mobile phase with a flow rate of 0.9 mL/min. Sodium diclofenac was selected as internal standard. Excellent linearity between the peak area ratios and losartan concentrations over the range of 2-200 ng/mL of plasma was observed. The limit of determination with UV detection at 225 nm, with a CV < 5% was 2 ng/mL in 500 L of plasma sample. The assay was rapid, safe and reliable for use in pharmacokinetic studies of losartan in human being.
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Volume 2, Issue 1 (5-2003) Back to browse issues page