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Detection of Ureaplasma Urealyticum in Clinical Samples from Infertile Women by Polymerase Chain Reaction
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Shahin Najar Peerayeh   , Roghayeh Samimi |
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Abstract: (1547 Views) |
| Genital Ureaplasma urealyticum infection is considered to be a sexually transmitted infection. The bacterium has been found to be involved in PID, chorioamnionitis, urethritis, respiratory distress syndrome and pneumonia in newborn, abortion and infertility. U. urealyticum infections not only jeopardize fertility but also pose a risk for infertility treatment and resulting pregnancies. The purpose of this study was to determine the prevalence of U. urealyticum in specimens from infertile women by polymerase chain reaction (PCR). 377 endocervical swab samples were taken from infertile women. Mycoplasma genus and U. urealyticum were detected by means of the polymerase chain reaction with specific primers. Mycoplasma genus DNA was detected in specimens from 141 (37.4%) of 377 patients. Of these 141 patients 85 (22.5% of total specimens) were PCR positive with urease gene primers for U. urealyticum. The isolation rate of U. urealyticum in young women (<30 age) was higher than others. Because of the potential adverse effects of U. urealyticum on the success rate of highly specialized infertility treatment, and its causal roles in several maternal complications of pregnancy and in neonatal morbidity and mortality, the rapid detection of U. urealyticum by PCR in infertile women could be important and necessary. The increased sensitivity and shorter time requirement of PCR support its further development for the diagnosis of U. urealyticum infections. |
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Full-Text [PDF 157 kb]
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Article Type: Research Article |
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