2024-03-28T13:49:01+04:30 http://ijpt.iums.ac.ir/browse.php?mag_id=25&slc_lang=en&sid=1
25-272 2024-03-28 10.1002
Iranian Journal of Pharmacology and Therapeutics 3 9 10 7 2015 13 1 Effect of short and long term intake of traditional aphrodisiac Cantharis Q on liver of male albino rat Roshan Clarence D'Souza Raghunandan P. Athalye Cantharis Q has a long history in both folk and traditional medicine being used as an aphrodisiac. It is a crude alcoholic extract of blister beetle Lytta vesicatoria commonly known as Spanish fly having cantharidin, a venomous substance present in it. The aim of the present study was to evaluate the clinical application of Cantharis Q by assessing its effect on the vital organ, liver, for a short and long period in male albino rat. The recovery study was also carried out in order to establish the reversibility of the treatment. Male albino rats of Wistar strain were given daily oral dosage of 500 ?g/Kg body weight Cantharis Q for 15 and 60 days. One group of 60-day treated rats was then kept for recovery without dosing for 30 days. The biochemical analysis of liver showed that the activity of alkaline and acid phosphatase enzymes decreased in the treated rats for both durations of treatment. Livers of 15-day treated animals had significantly increased RNA and total protein. Sixty days treatment led to a significant increase in cholesterol and significant decrease in glycogen, total protein and RNA levels in liver. Recovery animals had liver glycogen content significantly depleted whereas cholesterol, total protein and RNA increased significantly. The histopathology showed loss of cytoarchitecture in various areas of liver, vacuolation and inflammatory cellular infiltration in all the groups. Thus, it was observed that oral intake of Cantharis Q had a negative impact on the hepatic function of male rats and the recovery was also not found to be significant. 2015 8 01 1 0 http://ijpt.iums.ac.ir/article-1-272-en.pdf
25-271 2024-03-28 10.1002
Iranian Journal of Pharmacology and Therapeutics 3 9 10 7 2015 13 1 Comparative Evaluation of in Vitro effects of Praziquantel (PZQ) on the Enzyme Activities of the Excretory - Secretory Products (ESP) of Fasciola hepatica and F. gigantica Parasites Ali Farahnak Taghi Golmohamdi Mohamad Reza Eshraghian Excretory-secretory products (ESP) play an important role in the host biochemical defense by means of activities of detoxifying and antioxidant enzymes. The aim of this study was to evaluate praziquantel (PZQ) effects by detection of glutathione S- transferase (GST) and superoxide dismutase (SOD) enzymes activities in ESP samples of Fasciola spp. Fasciola gigantica and Fasciola hepatica adult parasites were collected and cultured within buffer media for 4 h at 37°C. Treated (with 50, 100 and 150 mg PZQ) and control ESP samples for each species were collected and centrifuged. The supernatants were stored at -20 ° C. SOD and GST enzymes activities of ESP samples were estimated photometrically. To determine the statistically-significant difference between ESP of treated and control samples, t-test was conducted. ESP protein bands were detected by gel electrophoresis (SDS-PAGE). SOD enzyme activities level in treated F.hepatica and F. gigantica ESP samples were determined as 0.26, 0.29, 0.76 and 1.73, 1.60, 1.43 U/ml respectively. SOD activities level in control samples were detected 1.31 and 1.35 U/ml. GST activities level in treated F.hepatica and F. gigantica ESP samples were calculated 9.4, 3.1, 23.96 and 435.4, 333.3, 720.9 U/ml. GST activities levels in control samples were detected 43.8 and 134.4 U/ml respectively. Statistical analysis revealed the significant decrease of GST and SOD enzyme activities in treated ESP samples of Fasciola hepatica , and significant increase of GST enzyme activity in treated ESP samples of F. gigantic a in comparison to the control samples ( p < 0.05). There was also no difference between SDS-PAGE results of treated and control samples. Based on the results of present work, PZQ has decreasing effect on the ESP enzymatic activities of Fasciola hepatica and increasing effect on F. gigantica enzyme activities. In other words, F. hepatica has less capability to protect against xenobiotics and free radicals than F.gigatica does. 2015 8 01 9 0 http://ijpt.iums.ac.ir/article-1-271-en.pdf
25-269 2024-03-28 10.1002
Iranian Journal of Pharmacology and Therapeutics 3 9 10 7 2015 13 1 Wound Healing Activity of Mimusops elengi Leaves Swapna Aleti M. Swapna Reddy J. A. Sneha N. V. L. Suvarchala In the present investigation, methanolic extract of Mimusops elengi  leaves were evaluated for wound healing activity. Entire wound healing process is complex series of events that begins at the moment of injury and can continue for months to years. Methanolic extract of Mimusops elengi leaves were examined for wound healing activity in the form of ointment by the excision, the incision and dead space wound model in mice. The extract ointments showed considerable wound healing properties with excision wound model ( p <0.001), incision wound model ( p <0.05), and dead space wound model ( p <0.05) when results were compared with control. Standard used for the present study was Betadine ointment in terms of wound contracting ability, wound closure time, tensile strength and dry granuloma weight. Results obtained clearly indicated that methanolic extract of Mimusops elengi  leaves exhibit significant wound healing properties. 2015 8 01 13 0 http://ijpt.iums.ac.ir/article-1-269-en.pdf
25-270 2024-03-28 10.1002
Iranian Journal of Pharmacology and Therapeutics 3 9 10 7 2015 13 1 Studies of Cytotoxic Potential of Aqueous Fruit Extract of Cucumis melo.Linn in Prostate Cancer Cell lines PC-3 Using MTT and Neutral Red Assay Sibi Perumbamkudiyil Ittiyavirah Ann George Anju M. Santhosh Sudhi T. Kurian Prinsy Pappachan Gifty Jacob The objective of the study was to evaluate the cytotoxic effects of aqueous extract of Cucumis melo fruit on human prostate cancer cell line (PC-3) using MTT and neutral red assays. The crude aqueous extract of C. melo was prepared by cold maceration method, filtered, concentrated and tested on PC-3 cell line. Dose-dependent cytotoxic activities were exhibited on human prostate carcinoma PC-3 cell line. As the dose of the extract increased, the number of viable cells decreased. This confirms the cytotoxic potential of the fruit of C. melo. 2015 8 01 19 0 http://ijpt.iums.ac.ir/article-1-270-en.pdf
25-268 2024-03-28 10.1002
Iranian Journal of Pharmacology and Therapeutics 3 9 10 7 2015 13 1 Adverse drug reactions causing admission to a Pediatric Hospital in Hamedan – Iran: a 2 years study Mojgan Safari Zahra Zandian Sima Vakili Bahman Rasuli Severe adverse drug reactions are an important cause of childhood morbidity and mortality. Despite this, few reports in the literature describe it. This study was designed to evaluate the admitted pediatric patients due to adverse drug reactions. This retrospective study was conducted at Pediatric ward of Besat Hospital. The records of hospitalized patients that admitted with diagnosis of adverse drug reactions enrolled in study over a 2 years period. The data collected on a data collection form using the records of the patients and included the patient demographic features (age, gender, allergy history, primary cause for drug administration) and drug information (name, duration of use, description of drug reaction, duration of drug reaction and concomitant use of drugs). Thirty-five patients were enrolled in study. Of them, 24 (68.6%) were male and 11 (31.4%) were female. Ages were under 1 years old in 5 (14%), between 1 to 5 years old in 22 (63%) and beyond 5 years old in 8 (23%) patients. Most patients (88.5%) had no known drug allergies. The primary cause for drug administration was upper respiratory tract infections (43%), diarrhea (26%), seizure (14%), gastroenteritis (8.5%) and vomiting (8.5%). Drugs most frequently cited included: Furazolidone (n = 9), Penicillin (n = 8), Amoxicillin (n = 7), Co-Trimoxazole (n = 4) and Phenobarbital (n = 4). Most frequent reactions were: skin rashes (83%), arthralgia (37%), fever (34%), pruritus (23%) and edema (11.5%). Understanding causality and preventability of adverse drug reactions will significantly aid in better therapeutic monitoring for children receiving the same therapy in future. 2015 8 01 26 0 http://ijpt.iums.ac.ir/article-1-268-en.pdf
25-267 2024-03-28 10.1002
Iranian Journal of Pharmacology and Therapeutics 3 9 10 7 2015 13 1 Hypoglycemic Activity of Inflorescence of Borassus flabellifer Extracts on Blood Glucose Levels of Streptozocin-Induced Diabetic Rats Pradeep Goyal M. Pharm Nilesh Jain G. K. Singh B. P. Nagori Evaluation of hypoglycemic activity of inflorescence of Borassus flabellifer extract in male wistar rats has been investigated. Antidiabetic potentials were studies by the orally administration of plant extract at the doses of 150, 300 and 600 mg/kg in streptozocin-induced diabetic male wistar rats. Treatment of streptozocin-induced diabetic male wistar rats with the extracts caused a significant ( p <0.01) reduction in the blood glucose levels when compared with control. The dose of 600 mg/kg showed a significant decrease in blood sugar ( p <0.05) after 2, 4, 6 and 8 hours of extracts administration when compared to control normal saline.  The highest activity resided at the dose of 600 mg/kg with mean percentage glycemic change of 39.50% after 8 hours of extract administration while the other two doses 150 and 300 mg/kg had glycemic change of 30.34% and 31.42% respectively after 8 hours of extract administration. The phytochemical screening revealed the presences of tannins, carbohydrate, terpenes, saponins, flavonoids and alkaloids. This result suggests that the inflorescence of Borassus flabellifer extracts possess antidiabetic effect on streptozocin-induced diabetic male wistar rats. 2015 8 01 30 0 http://ijpt.iums.ac.ir/article-1-267-en.pdf
25-266 2024-03-28 10.1002
Iranian Journal of Pharmacology and Therapeutics 3 9 10 7 2015 13 1 Effect of Citrus Medica Linn. in Urolithiasis Induced by Ethylene Glycol Model Avani Pushkar Shah Snehal Patel Kirti Patel Tejal Gandhi The Objective of this study is to study the Effect of Citrus medica in urolithiasis induced by Ethylene Glycol model. A number of 24 Male Wistar albino rats (250-300 g) were divided in to 4 groups namely Group I (Normal control), Group II (Model control), Group III (Standard-treated, cystone – 750 mg/kg) and Group IV (Citrus medica – 250 mg/kg). Except group I, Ethylene glycol (0.75% v/v in drinking water) was provided for each group of animals for 28 days study period. After the completion of treatment, various physical parameters (body weight, diuresis, pH), level of urolithiatic promoters (calcium, oxalate, Inorganic phosphate and uric acid) and urolithiatic inhibitors (magnesium and citrate) were measured in 24-h urine, blood samples and kidney homogenate. Antioxidant parameters & renal function tests were carried out as well. Both cystone and extract of Citrus medica showed significant increase in physical parameters and stone forming inhibitors and significant decrease in stone forming promoters. Degree of oxidative stress reduced with cystone and in treatment group. Extract of Citrus medica Linn. showed significant activity against urolithiasis which is due to diuretic and antioxidant activity and its ability to increase inhibitors and decrease promoters levels. 2015 8 01 35 0 http://ijpt.iums.ac.ir/article-1-266-en.pdf
25-265 2024-03-28 10.1002
Iranian Journal of Pharmacology and Therapeutics 3 9 10 7 2015 13 1 Evaluation of Diuretic and Antidiabetic Activity of Esculin E. Venkateshwarlu B. S. Sharvanabhava P. Dileep A. K. Kaleem M. D. Arif E. Rajeev Reddy S. Achyuth Bharadwaj Diuretics are the agents which cause increase in excretion of urine. Diabetes mellitus (DM) is a multi-factorial disorder characterized by hyperglycemia resulting from an increased hepatic glucose production and diminished insulin secretion. The present study was undertaken to study the diuretic and anti-diabetic activity of esculin. Esculin was found to have potent diuretic activity in normal rats and the potency was comparable to that of standard drug furosemide. The diuretic effects of both concentrations of the drug were indicated by increase in water, sodium and potassium excretion. Esculin also at different doses produced a significant fall in the blood glucose level in both normal and diabetic rats in a dose-dependent manner which was evident 4 h after the administration of the drug. Among the two doses of test drug (100 and 200 mg/kg), the later dose showed significant anti-hyperglycemic activity. 2015 8 01 40 0 http://ijpt.iums.ac.ir/article-1-265-en.pdf