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Literature & Humanities
http://ijpt.iums.ac.ir
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admin
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8888
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en
jalali
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gregorian
2018
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Toxicity of nano and bulk forms of cerium oxide in different cell lines
Toxicology
پژوهشي
Research Article
In recent years, nanotechnology has gained serious attention for diagnosis, prevention and treatment roles. In this study we synthesized nanoceria or CeO<sub>2</sub>NPs (cerium oxide nanoparticles) and compared toxicity of cerium oxide powder in nano and bulk forms in two cancerous and one normal cell lines. The cell lines were cultured in a standard humidified incubator, at 37 °C in a 5% CO<sub>2</sub> atmosphere, in RPMI 1640 medium. The cells were incubated with different concentrations of cerium oxide (from 2 μg/mL to 64 μg/mL) in bulk and nano forms. To determine the effect of cerium oxide on cell viability after 24 h, 48 h, and 72 h incubation, a MTT assay was performed using <em>SKBR3 </em><em>(</em>human breast cancer cell line<em>), </em>A431 (Human epidermoid carcinoma cell line) and C2Cl2 (ATCC mouse skeletal muscle cell line) cells. Analysis of variance followed by Sidak post-hoc test, shows the toxicity of nanoceria is significantly deferent from bulk form on three cell lines in this study and is more on cancerous cells in compared to normal cells especially in higher level of concentrations after 24, 48 and 72 hours (All P<0.05). Additionally, the effect of cell lines, cerium oxide forms and concentrations cerium oxide leads in significantly the lowest amount of viability after 72 hours compared with 24 hours and 48 hours.
Cerium oxide, Nanoceria, MTT assay, C2Cl2 cells, A431 cells, SKBR3 cells
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http://ijpt.iums.ac.ir/browse.php?a_code=A-10-38-1&slc_lang=en&sid=1
FATEMEH
SOLTANI
10031947532846001545
10031947532846001545
No
Department of Medical Radiation Engineering, Science & Research Branch, Islamic Azad University, Tehran, Iran
KAMAL
YAVARI
10031947532846001546
10031947532846001546
No
Radiopharmaceutical Research and Development Lab (RRDL), Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran
MAHDI
SADEGHI
sadeghi.m@iums.ac.ir
10031947532846001544
10031947532846001544
Yes
Medical Physics Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
ALI
BAHRAMI SAMANI
10031947532846001547
10031947532846001547
No
Radiopharmaceutical Research and Development Lab (RRDL), Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran
SIMINDOKHT
SHIRVANI ARANI
10031947532846001548
10031947532846001548
No
Radiopharmaceutical Research and Development Lab (RRDL), Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran