2
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Literature & Humanities
http://ijpt.iums.ac.ir
1
admin
9
10
8
7
14
8888
13
en
jalali
1387
9
1
gregorian
2008
12
1
7
2
online
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fulltext
en
Glycosaminoglycans (GAG) from backwater clam Marcia opima (Gmelin)
پژوهشي
Research Article
Heparin was isolated from whole clam tissue Marcia opima . Purification of multimilligram quantities of heparin from this tissue sample permitted a thorough examination of its anticoagulant activity characterization, which was partially purified by fractionation by anion exchange chromatography using DEAE cellulose column. The isolated crude and partially purified fractionated sample showed metachromatic shift while using azure-A. The sample also exhibited prominent anticoagulant activity. Mobility was analyzed by two different buffer systems using agarose gel electrophoresis. The fractionated molluscan GAG was also found to have similar peaks as that of standard heparin when assessed by the FT-IR spectrum. The bivalve GAG was subjected to fractionation for further purification and its chemical components were analyzed. The fractionated clam heparin also showed substantial in-vitro anticoagulant activity compared to that of commercial heparins.
147
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http://ijpt.iums.ac.ir/browse.php?a_code=A-10-100-185&slc_lang=en&sid=1
Vijayabaskar
Pandian
1003194753284600617
1003194753284600617
No
Somasundaran
Thirugnanasambandan
1003194753284600618
1003194753284600618
No