[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 14, Issue 1 (11-2015) ::
3 2015, 14(1): 11-20 Back to browse issues page
Dual role of CdSe quantum dots for simultaneous separation and spectrofluorimetric ultrasensitive determination of heparin
BEHZAD TORABI , FARZANEH SHEMIRANI , ZAHRA AHMADI JOOZDANI , FARZAD TORABI
Department of Analytical Chemistry, University College of Science, University of Tehran, Tehran, Iran.
Abstract:   (219 Views)
The present study offers a new method based on CdSe quantum dots (QDs) for simultaneous separation and determination of trace levels of heparin (Hep) in human serum samples. In this technique, CdSe QDs perform two different functions in Hep analysis process. Mercaptoacetic acid-capped red CdSe QDs (λex=690 nm) are conjugated to Hep and the Hep-QD conjugation is then used as an extraction tool from microencapsulation of real samples. For quick release of the analyte from conjugation, the microcapsules are irradiated using an intensive near infrared wavelength as the controllable releasing agent. The L-cysteine-capped green CdSe QDs (λex=480 nm) play the additional role of Hep spectrofluorimetric detection. By labeling the appropriate chromophore, the extracted Hep can effectively quench green CdSe QD fluorescence which is quenched relative to Hep concentration. In comparison with other available methods, the newly-developed assay has a low detection limit (0.3 nmol L-1), wide linear range (1 to 8500 nmol L-1), good accuracy and high selectivity, that make it a good candidate for Hep separation and monitoring of complex physiological samples.
Keywords: Heparin, Quantum dot, Determination, Human serum, Near infrared
Full-Text [PDF 644 kb]   (74 Downloads)    
Article Type: Research Article | Subject: Absorption, Distribution, Metabolism and Excretion
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

TORABI B, SHEMIRANI F, AHMADI JOOZDANI Z, TORABI F. Dual role of CdSe quantum dots for simultaneous separation and spectrofluorimetric ultrasensitive determination of heparin. 3. 2015; 14 (1) :11-20
URL: http://ijpt.iums.ac.ir/article-1-295-en.html


Volume 14, Issue 1 (11-2015) Back to browse issues page
مجله داروشناسی و درمان شناسی ایران Iranian Journal of Pharmacology and Therapeutics
Persian site map - English site map - Created in 0.3 seconds with 33 queries by YEKTAWEB 3760