[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 2, Issue 1 (5-2003) ::
3 2003, 2(1): 18-0 Back to browse issues page
A High-Performance Liquid Chromatographic Assay for the Determination of Losartan in Plasma
Hassan Jalalizadeh , Effat Souri , Hassan Farsam , Mehdi Ansari
Abstract:   (138 Views)
A rapid and sensitive HPLC method was developed for determination of losartan in plasma. Losartan was extracted from plasma by a two-step extraction procedure using chloroform as extracting solvent in acidic medium. HPLC analysis was performed on a cyano reversed-phase column using phosphate buffer (pH 4.3), acetonitrile (750:250, v/v) as mobile phase with a flow rate of 0.9 mL/min. Sodium diclofenac was selected as internal standard. Excellent linearity between the peak area ratios and losartan concentrations over the range of 2-200 ng/mL of plasma was observed. The limit of determination with UV detection at 225 nm, with a CV < 5% was 2 ng/mL in 500 L of plasma sample. The assay was rapid, safe and reliable for use in pharmacokinetic studies of losartan in human being.
Full-Text [PDF 327 kb]   (70 Downloads)    
Article Type: Research Article |
Add your comments about this article
Your username or Email:

CAPTCHA code


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Jalalizadeh H, Souri E, Farsam H, Ansari M. A High-Performance Liquid Chromatographic Assay for the Determination of Losartan in Plasma. 3. 2003; 2 (1) :18-0
URL: http://ijpt.iums.ac.ir/article-1-18-en.html


Volume 2, Issue 1 (5-2003) Back to browse issues page
مجله داروشناسی و درمان شناسی ایران Iranian Journal of Pharmacology and Therapeutics
Persian site map - English site map - Created in 0.23 seconds with 33 queries by YEKTAWEB 3731