[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 7, Issue 2 (12-2008) ::
3 2008, 7(2): 147-0 Back to browse issues page
Glycosaminoglycans (GAG) from backwater clam Marcia opima (Gmelin)
Vijayabaskar Pandian , Somasundaran Thirugnanasambandan
Abstract:   (117 Views)
Heparin was isolated from whole clam tissue Marcia opima . Purification of multimilligram quantities of heparin from this tissue sample permitted a thorough examination of its anticoagulant activity characterization, which was partially purified by fractionation by anion exchange chromatography using DEAE cellulose column. The isolated crude and partially purified fractionated sample showed metachromatic shift while using azure-A. The sample also exhibited prominent anticoagulant activity. Mobility was analyzed by two different buffer systems using agarose gel electrophoresis. The fractionated molluscan GAG was also found to have similar peaks as that of standard heparin when assessed by the FT-IR spectrum. The bivalve GAG was subjected to fractionation for further purification and its chemical components were analyzed. The fractionated clam heparin also showed substantial in-vitro anticoagulant activity compared to that of commercial heparins.
Full-Text [PDF 754 kb]   (60 Downloads)    
Article Type: Research Article |
Add your comments about this article
Your username or Email:

Write the security code in the box >


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Pandian V, Thirugnanasambandan S. Glycosaminoglycans (GAG) from backwater clam Marcia opima (Gmelin). 3. 2008; 7 (2) :147-0
URL: http://ijpt.iums.ac.ir/article-1-175-en.html
Volume 7, Issue 2 (12-2008) Back to browse issues page
مجله داروشناسی و درمان شناسی ایران Iranian Journal of Pharmacology and Therapeutics
Persian site map - English site map - Created in 0.14 seconds with 33 queries by YEKTAWEB 3647